Journal: Materials Today Bio
Article Title: Matrix-bound EGF promotes malignant phenotypes of breast cancer organoids in the biomimetic ECM of alginate
doi: 10.1016/j.mtbio.2025.101818
Figure Lengend Snippet: Western blots of PI3K-AKT pathway showing expression of STAT6 and p-STAT6 (A), ERK1/2 and p-ERK1/2 (B), PI3K, p-PI3K, AKT, p-AKT, mTOR and p-mTOR (C) and ratios of the activated protein to the total protein were calculated. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, n = 3, analyzed by two-tailed Student's t-test.
Article Snippet: For Western Blot, anti-GAPDH (5174T), anti-ERK (4695T), anti- (phosphor) -ERK (9472T), anti-STAT (4904T) anti- (phosphor) -STAT (9145T), anti-AKT (9272T), anti- (phosphor) -AKT (4048T), anti-mTOR (2983T) and anti- (phosphor) - mTOR (5536T) were purchased from Cell Signaling Technology (USA), anti-E-cadherin (ab314063), anti-vimentin (ab20346), anti-PI3K (ab191606) and anti- (phosphor)-PI3K (ab278545) were purchased from Abcam (USA).
Techniques: Western Blot, Expressing, Two Tailed Test